[Bioluminescent determination of NAD- and NADP-dependent lymphocytic glutamate dehydrogenases]

A sensitive bioluminescent rapid method for estimating the activity of pyridine nucleotide-(phosphate)-dependent glutamate dehydrogenases (NAD(P)-GDH) of human peripheral blood lymphocytes is suggested. 10000 cells is sufficient per analysis, up to 100 measurements may be made within 3 hrs. Normal subjects and subjects often suffering from acute respiratory diseases were examined for the aforesaid parameter. The findings evidence the diagnostic significance of NAD(P)-GDH for the detection of subjects at risk of developing acute respiratory diseases.