Origin of the anomalous two-photon absorption in fluorescent protein DsRed
1NEST-CNR INFM and Scuola Normale Superiore, Piazza dei Cavalieri 7, I-56126 Pisa, Italy. r.nifosi@sns.it
Abstract:
The red fluorescent protein DsRed displays a two-photon excitation band around 760 nm which is not accompanied by any feature in the corresponding one-photon spectral region (380 nm). By means of time-dependent density functional theory, we are able to explain such an effect, as arising from an electronic excitation of the DsRed chromophore with ability to couple with a charge-transfer state, through an effective two-photon absorption channel.
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