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Redox potential measurement as a rapid method for microbiological testing and its validation for coliform
O Reichart1, K Szakmár, A Jozwiak
1Szent István University, Faculty of Veterinary Science, Department of Food Hygiene, H-1078 Budapest, István u. 2, Hungary. microtest1@t-online.hu
International Journal of Food Microbiology
|January 19, 2007
Summary
Measuring redox potential changes offers a reliable method for quantifying microbial contamination. The time to detection (TTD) correlates linearly with microbial concentration, simplifying cell count determination.
Area of Science:
- Microbiology
- Biotechnology
- Analytical Chemistry
Background:
- Redox potential is a complex indicator of microbial physiological status.
- Monitoring redox potential can aid in microbial contamination assessment.
- Bacterial growth leads to a decrease in medium redox potential.
Purpose of the Study:
- To explore the utility of redox potential measurement for microbial determination.
- To establish a correlation between redox potential changes and microbial concentration.
- To develop and validate a novel method for microbial quantification.
Main Methods:
- Development of a computer-controlled multi-channel system for redox potential measurement.
- Defining Time to Detection (TTD) based on significant redox potential change.
- Establishing a calibration curve correlating TTD with the logarithm of initial microbial concentration.
- Validation of the method for coliform bacteria determination.
Main Results:
- The rate of redox potential change (dE/dt) is proportional to living cell concentration.
- A strict linear correlation was found between TTD and the logarithm of initial microbial concentration.
- The developed method demonstrated high efficiency and reliability for coliform bacteria detection.
Conclusions:
- Redox potential measurement provides a simplified approach for determining living cell concentration.
- The novel method is efficient and reliable for qualitative and quantitative microbial analysis.
- This technique offers a valuable tool for assessing microbial contamination in cultures.
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