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Related Experiment Videos

A comparative study on commercial samples of phellodendri cortex.

Y M Liu1, S J Sheu, S H Chiou

  • 1Department of Chemistry, National Taiwan Normal University, 88 sec. 5, Roosevelt Road, Taipei, Taiwan, Republic of China.

Planta Medica
|December 1, 1993
PubMed
Summary
This summary is machine-generated.

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Commercial Phellodendri cortex samples from Taiwan and Japan were analyzed for alkaloid content. Phellodendron wilsonii and P. amurense var. sachalinense showed higher alkaloid levels, with berberine being the predominant compound.

Area of Science:

  • Pharmacognosy
  • Phytochemistry
  • Analytical Chemistry

Background:

  • Phellodendri cortex, derived from Rutaceous plants, is a traditional herbal medicine.
  • Variations in Phellodendri cortex quality and origin can impact its therapeutic efficacy.
  • Accurate identification and quality assessment of Phellodendri cortex are crucial for its medicinal use.

Purpose of the Study:

  • To determine the alkaloid profiles of commercial Phellodendri cortex samples.
  • To differentiate between Phellodendron species and varieties based on chemical constituents.
  • To establish a method for assessing the origin and quality of Phellodendri cortex.

Main Methods:

  • Collection of 31 commercial Phellodendri cortex samples from Taiwan and Japan.
  • Analysis of five quaternary alkaloids (berberine, palmatine, jatrorrhizine, phellodendrine, magnoflorine) using capillary electrophoresis.

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  • Evaluation of alkaloid content and comparison across different Phellodendron species and varieties.

    • Main Results:

    • Phellodendron wilsonii and P. amurense var. sachalinense exhibited significantly higher total alkaloid content (approx. 4.1%) compared to P. amurense and P. chinense (approx. 1.5%).
    • Berberine was the major alkaloid, constituting about 80% of total alkaloids in P. wilsonii and P. amurense var. sachalinense, but only about 40% in P. amurense and P. chinense.
    • Chemical analysis data, combined with herb texture and color, can help postulate the origin and quality of Phellodendri cortex.

    Conclusions:

    • Phellodendron wilsonii and P. amurense var. sachalinense are superior sources of Phellodendri cortex based on alkaloid content.
    • Berberine content can serve as a key indicator for differentiating Phellodendri cortex quality and origin.
    • Capillary electrophoresis is an effective method for the quantitative analysis of alkaloids in Phellodendri cortex, aiding in quality control.