Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse inbred strain differences in ethanol drinking to intoxication.

J S Rhodes1, M M Ford, C-H Yu

  • 1Department of Psychology, University of Illinois at Urbana-Champaign, Champaign, IL, USA. jrhodes@uiuc.edu

Genes, Brain, and Behavior
|January 20, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Results on the Search for Lepton Number Violating Neutrinoless Double-β Decay with the LEGEND-200 Experiment.

Physical review letters·2026
Same author

Final Results of the Majorana Demonstrator's Search for Double-Beta Decay of ^{76}Ge to Excited States of ^{76}Se.

Physical review letters·2025
Same author

Evidence of Coherent Elastic Neutrino-Nucleus Scattering with COHERENT's Germanium Array.

Physical review letters·2025
Same author

Surgical management of unilateral restrictive strabismus in an 8-month-old dog.

New Zealand veterinary journal·2024
Same author

Exotic Dark Matter Search with the Majorana Demonstrator.

Physical review letters·2024
Same author

Measurement of Electron-Neutrino Charged-Current Cross Sections on ^{127}I with the COHERENT NaIνE Detector.

Physical review letters·2023
Same journal

Dissecting the Strain and Sex Specific Connectome Signatures of Unanesthetized C57BL/6J and DBA/2J Mice Using Magnetic Resonance Imaging.

Genes, brain, and behavior·2026
Same journal

Exploring the Association Between Mental Disorders and Various Arrhythmias via Mendelian Randomization Methods.

Genes, brain, and behavior·2026
Same journal

Genetic Architecture of Addiction-Relevant Behaviors in Outbred Sprague-Dawley Rats Reveals Loci for Anxiety-Like and Nociceptive Traits.

Genes, brain, and behavior·2026
Same journal

∆FOSB in the Nucleus Accumbens Core Is Required for Increased Anxiety, but Not Decreased Social Motivation, Following Estrogen Withdrawal in Female Mice.

Genes, brain, and behavior·2026
Same journal

Taste Preferences Shape Disease Susceptibility via Heritable Nutrient Predispositions: A Behavior-Gene Coevolution Framework.

Genes, brain, and behavior·2026
Same journal

Whisking Behaviour Reveals Stronger Evidence of Habituation in Homozygous Reeler Mice Compared to Controls.

Genes, brain, and behavior·2026
See all related articles

The Drinking in the Dark (DID) model allows mice to self-administer ethanol, reaching high blood ethanol concentrations (BECs). This method reveals genetic influences on ethanol intake and its correlation with other traits.

Area of Science:

  • Neuroscience
  • Genetics
  • Pharmacology

Background:

  • The Drinking in the Dark (DID) procedure enables mice to self-administer ethanol.
  • This method achieves high blood ethanol concentrations (BECs) above 1 mg/ml in C57BL/6J mice.
  • DID involves replacing water with 20% ethanol for 4 hours during the dark phase.

Purpose of the Study:

  • To further explore the high ethanol drinking model (DID).
  • To analyze the behavioral patterns of ethanol intake in DID.
  • To investigate the genetic influences on DID and its correlation with other traits.

Main Methods:

  • Microanalysis of C57BL/6J mouse behavior during DID.
  • Assessment of DID-impaired performance on the accelerating rotarod and balance beam.

Related Experiment Videos

  • Screening of 12 inbred mouse strains to evaluate genetic factors in DID and related traits.

    • Main Results:

    • Ethanol drinking patterns in DID differ from routine water intake.
    • DID significantly impairs motor coordination and balance in mice.
    • Significant genetic variations in ethanol intake and BEC were observed across strains, with C57BL/6J showing the highest levels.
    • Strain means correlated positively with ethanol intake and BEC in standard and limited two-bottle tests, with DID yielding higher BECs.
    • Genetic correlations of high ethanol drinking with low ethanol withdrawal severity and taste aversion were confirmed, even after phylogenetic correction.

    Conclusions:

    • The DID model provides a robust method for studying high ethanol consumption in mice.
    • Genetic factors significantly influence ethanol intake and BEC levels in the DID model.
    • DID is a valuable tool for identifying genes associated with pharmacologically significant ethanol consumption and related behaviors.