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Updated: Jul 17, 2026

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
Published on: December 9, 2022
Role of activity-dependent regulation of neuronal chloride homeostasis in development
Hubert Fiumelli1, Melanie A Woodin
1Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, Rue du Bugnon 7, CH-1005 Lausanne, Switzerland.
Abstract:
The polarity of neurotransmission mediated by the gamma-amino butyric acid (GABA) type A receptor depends crucially on intracellular chloride concentration, which is largely determined by the expression and function of cation/chloride co-transporters. Recent evidence shows how both activity and neurotrophic factors can affect GABAergic transmission in the mammalian central nervous system through their effects on the neuron-specific chloride-extruding transporter KCC2. In particular, GABAergic neurotransmission early in development, sustained neuronal activity in mature networks and brain-derived neurotrophic factor each modulate the expression or function of KCC2. The resulting changes in intracellular chloride concentration alter the nature or strength of fast GABAergic neurotransmission, profoundly affecting the development and function of neuronal networks.
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