Massive, sustained gammadelta T cell migration from the bovine skin in vivo

In all species studied so far, gammadelta T cells are abundantly present in epithelia. The functions of these cells are largely unknown. Using a lymph duct cannulation method, which is only possible in large animals such as cattle, we show that large numbers of gammadelta T cells, but not alphabeta T cells, are constitutively present in pseudoafferent lymph draining bovine skin. The gammadelta T cells, which are present in pseudoafferent lymph, use Vgamma segments that are characteristic for bovine dermal gammadelta T cells, suggesting that these cells migrated from the skin. Further supporting the origin of these cells is the fact that fluorescent latex beads injected in the skin could be recovered in cells in the pseudoafferent lymph. The cannulation method is minimally invasive, and the lymph flow, which was sustained and remained essentially unaltered during 14 days, closely represents the in vivo situation. The gammadelta T cells could not be induced to produce IFN-gamma, TNF-alpha, and IL-10, and they did not express costimulatory molecules, IL-2 receptor, and MHC Class II molecules. The level of gammadelta T cell egress was 6.7x10(3) gammadelta T cells per cm2 skin per hour, which is enough to deplete all gammadelta T cells from the skin within 46 h. As this massive gammadelta T cell migration was observed during 14 days, constant replenishment of these cells must take place. Our data suggest that gammadelta T cells in tissues fulfill more than exclusively local functions.