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Updated: Jul 17, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Bioactive Diterpenoids Isolated from Dilophus ligulatus.

N Bouaïcha¹, C Tringali, D Pesando

¹INSERM U303 "Mer et Santé" B.P. 3, F-06230 Villefranche-sur-mer, France.

|June 1, 1993

Summary

Nine diterpenoids from the brown alga Dilophus ligulatus were isolated and tested for bioactivity. Some compounds showed significant antifungal and cytotoxic effects, outperforming a standard control.

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Area of Science:

Marine Natural Products Chemistry
Phytochemistry
Pharmacology

Background:

The brown alga Dilophus ligulatus is a source of diverse secondary metabolites.
Diterpenoids are a class of natural products with a wide range of biological activities.

Purpose of the Study:

To isolate and characterize diterpenoids from Dilophus ligulatus.
To evaluate the antifungal and cytotoxic activities of the isolated compounds.

Main Methods:

Isolation of compounds using chromatographic techniques.
Structure elucidation via spectroscopic methods (Mass, IR, 1H-NMR, 13C-NMR).
Bioactivity screening including antifungal and cytotoxic assays.

Main Results:

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Published on: August 16, 2018

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Last Updated: Jul 17, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Nine diterpenoids were isolated and identified: acetyldictyolal (1), epoxyoxodolabelladiene (2), dictyotalide B (3), neodictyolactono (4), pachylactone (5), acetals 6A and 6B, isoacetoxycrenulatin (7), and dictyolactone (8).

Compounds 1, 2, 4, 7, and 8 demonstrated antifungal activity.

Eight diterpenoids were tested for cytotoxic activity against mammalian cell lines (KB, P-388, P-388/DOX, NSCLCN6-L16), with some exhibiting greater potency than the positive control, mercaptopurine.

Conclusions:

Dilophus ligulatus yields bioactive diterpenoids with potential pharmaceutical applications.
The isolated compounds possess promising antifungal and cytotoxic properties.
Further investigation into these diterpenoids could lead to the development of new therapeutic agents.