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Published on: April 24, 2021
Specific suppression of beta-secretase gene expression by short interfering RNA in SK-N-SH cells
Wei-Jiang Dong1, Gai-Feng Feng, Hui-Lin Gong
1Department of Anatomy and Histology and Embryology, Xi'an Jiaotong University Medical School, Xi'an 710061, China.
Objective:
To test the effect of short interfering RNAs (siRNAs) of beta-site APP cleaving enzyme (BACE) on inhibiting the expression of BACE in mammalian cells.
Methods:
The gene of EGFP, U6 promoter and beta-secretase targeting siRNA were cloned by PCR. The PCR products were inserted into the retrovirus plasmid pLXSN. The interfering vector was identified as pLXSN/ EGFP-U6-siBACE. The SK-N-SH cell line was produced, which can highly expressed BACE. The inhibitive effect of BACE siRNA on BACE expression was examined by fluoroscopy and immunohistochemistry tests.
Results:
The interfering vector, pLXSN/EGFP-U6-siBACE, was constructed successfully. The BACE siRNA inhibited the expression of BACE in the SK-N-SH cell and reduced the production of Abeta.
Conclusion:
BACE siRNA inhibits the expression of BACE gene of mammalian, which has implications for RNA interference of Alzheimer's disease.
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