Evolution of the "titin epitope" in neurofilament proteins
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Updated: Sep 21, 2026

Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
Published on: February 10, 2016
C Mencarelli1, B Magi, B Marzocchi
1Department of Evolutionary Biology, University of Siena, Italy.
Abstract:
1. The specificity of a monoclonal antibody raised to human titin was characterized. The antibody reacts with an epitope which is common to titin and the high mol. wt subunits NF-H and NF-M of mammalian neurofilaments. 2. Mapping of the epitope indicated that it is located in the carboxyterminal extension of NF-H and NF-M, and that its reactivity does not depend on the phosphorylation state of the molecule. 3. A comparative study on neurofilament protein of lower vertebrates revealed that this epitope has been conserved during vertebrate evolution.
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