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Human complement regulatory proteins expressed on mouse A9 cells containing a human chromosome 1
1Department of Immunology, Center for Adult Diseases Osaka, Higashinari-ku, Japan.
Immunology
|December 1, 1991
Summary
Human complement regulatory proteins on chromosome 1 protect mouse cells from complement activation. Transferring chromosome 1 to mouse cells enabled expression of these proteins, suppressing complement activity.
Area of Science:
- Immunology
- Genetics
- Cell Biology
Background:
- The human complement system is crucial for innate immunity but can target host cells.
- Complement regulatory proteins (CRPs) prevent self-attack by the complement system.
- The genes for key human CRPs are located on chromosome 1q3.2.
Purpose of the Study:
- To investigate the expression and function of human CRPs transferred into mouse cells.
- To determine if human CRPs can protect heterologous cells from complement-mediated damage.
Main Methods:
- Human chromosome 1 was transferred into mouse A9 fibroblasts (A9(neo-1) cells).
- Surface expression of CRPs (CR1, DAF, MCP, CR2) was assessed by flow cytometry.
- Complement activation and suppression were evaluated using human complement and A9(neo-1) cells.
Main Results:
- Human CRPs (CR1, DAF, MCP, CR2) were expressed on A9(neo-1) cells in a time-dependent manner, linked to chromosome 1.
- Mouse cells expressing human CRPs showed suppressed alternative pathway complement activation.
- Even with diminished MCP expression at 42 days, some protective activity remained.
Conclusions:
- Human chromosome 1 confers expression of functional complement regulatory proteins in mouse cells.
- Transferred human CRPs can protect heterologous cells from complement activation.
- This study demonstrates the potential of gene transfer for conferring complement resistance.