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A fast staining method for CNS slices
L Riboni1, F Luna, H Núñez-Durán
1Departamento de Ciencias Fisiológicas, Universidad Autónoma de Puebla, Mexico.
Journal of Neuroscience Methods
|July 1, 1991
Abstract:
We describe a staining technique for quick observation of the microanatomy of the vertebrate CNS. Successive soaking of the sections in tannic acid and ferric chloride produces a black colour in the gray matter. The procedure takes 5 min and is useful for identifying electrode penetrations, localization of lesions or teaching neuroanatomy.