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Updated: Jul 17, 2026

Generation of Zerovalent Metal Core Nanoparticles Using n-(2-aminoethyl)-3-aminosilanetriol
Published on: February 11, 2016
Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3
S Anil Kumar1, Majid Kazemian Abyaneh, S W Gosavi
1Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
Abstract:
Synthesis of silver nanoparticles using alpha-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.
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