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Molecular characterization of enteroviruses isolated from patients with aseptic meningitis in Korea, 2005

S-T Lee1, C-S Ki, N Y Lee

  • 1Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Archives of Virology
|January 24, 2007
PubMed

Insights

A 2005 Seoul aseptic meningitis outbreak was primarily caused by coxsackievirus B5 (CBV5) strains. Phylogenetic analysis revealed novel amino acid changes in CBV5 strains, suggesting potential antigenic significance.

Area of Science:

  • Virology
  • Epidemiology
  • Molecular Biology

Background:

  • Enteroviruses are primary causes of aseptic meningitis.
  • An aseptic meningitis outbreak occurred in Seoul, Korea, in 2005, linked to enterovirus strains.

Purpose of the Study:

  • To analyze clinical isolates from aseptic meningitis patients during the 2005 Seoul outbreak.
  • To identify and characterize the predominant enterovirus strains responsible for the outbreak.

Main Methods:

  • Serotyping using type-specific antisera.
  • Reverse transcription-polymerase chain reaction (RT-PCR).
  • Direct sequencing of 5' noncoding and VP1 regions.

Main Results:

  • Coxsackievirus B5 (CBV5) was the predominant strain (15/24 isolates).
  • Phylogenetic analysis of the VP1 region confirmed CBV5 predominance and grouped 12 strains with recent isolates from China and France.
  • Twelve CBV5 strains exhibited a novel amino acid change (AY to GN) in the BC-loop compared to the reference strain.

Conclusions:

  • Coxsackievirus B5 was the main etiological agent during the 2005 Seoul aseptic meningitis outbreak.
  • The identified amino acid changes in the CBV5 BC-loop may have antigenic implications and warrant further investigation.

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