Related Experiment Video
Updated: Jul 17, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Prototype integration of protein electrophoresis laboratory results in an information warehouse to improve workflow
Jianhua Liu1, Scott A Silvey, Michael G Bissell
1The Ohio State University Medical Center, Columbus, OH 43201, USA.
Abstract:
This poster demonstrates our efforts to enhance workflow and clinical analysis of protein electrophoresis (PEP) data through integration with the Information Warehouse (IW) at The Ohio State University Medical Center (OSUMC). A new desktop application has been developed with the aim of enabling more efficient and accurate gel analysis by clinical pathologists. This tool gives the pathologists the ability to perform their analysis conveniently from anywhere on the OSUMC network along with the aid of numerical analysis algorithms, image enhancement techniques, and access to historical PEP results for the given patient.
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

