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Updated: Jul 17, 2026

08:54
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Minimization of PCR efficiency differences between standards and samples through dilution of PCR amplicons in reverse
Marc Simard1, Eric Boucher, Pierre R Provost
1Ontogeny and Reproduction Unit, Centre Hospitalier Universitaire de Québec, Pavillon Centre Hospitalier de l'Université Laval, Sainte-Foy, Qué., Canada.
Analytical Biochemistry
|January 24, 2007
Abstract
No abstract available in PubMed .
Related Concept Videos
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
PCR
Overview

