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Minimization of PCR efficiency differences between standards and samples through dilution of PCR amplicons in reverse

Marc Simard1, Eric Boucher, Pierre R Provost

  • 1Ontogeny and Reproduction Unit, Centre Hospitalier Universitaire de Québec, Pavillon Centre Hospitalier de l'Université Laval, Sainte-Foy, Qué., Canada.

Analytical Biochemistry
|January 24, 2007
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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