Glucose biosensor based on nano-SiO2 and "unprotected" Pt nanoclusters

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biomedical Engineering

Background:

• Glucose biosensors are crucial for diabetes management.
• Developing efficient immobilization carriers enhances biosensor performance.
• Platinum nanoclusters and SiO2 nanoparticles offer unique properties for biosensor fabrication.

Purpose of the Study:

• To fabricate an amperometric glucose biosensor using Pt nanoclusters and SiO2 nanoparticles as an immobilization carrier for glucose oxidase (GOx).
• To evaluate the performance characteristics of the developed biosensor, including sensitivity, stability, detection limit, and operational pH range.

Main Methods:

• Preparation of a composite material from "unprotected" Pt nanoclusters (2 nm) and nanoscale SiO2 particles (13 nm).
• Immobilization of glucose oxidase (GOx) onto the Pt-SiO2 composite.
• Fabrication of an amperometric glucose biosensor.
• Characterization of biosensor performance using cyclic voltammetry and sensitivity measurements.

Main Results:

• The Pt-SiO2 composite effectively immobilized GOx while maintaining its bioactivity.
• The fabricated biosensor demonstrated high sensitivity (3.85 microA mM(-1)) and good stability.
• A low detection limit of 1.5 microM and a linear range from 0.27 to 4.08 mM were achieved.
• The biosensor operated effectively over a wide pH range (4.9-7.5).

Conclusions:

• The Pt-SiO2 composite is a promising carrier for GOx immobilization in amperometric glucose biosensors.
• The developed biosensor offers excellent sensitivity, stability, and a wide operational pH range, suitable for practical glucose monitoring.