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Published on: January 22, 2020
A processed metabolite of luteinizing hormone-releasing hormone has proliferative effects in endometrial cells
Kathryn Walters1, Yue Pui Chin, T John Wu
1Laboratory of Neuroendocrinology and Women's Health Research, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Objective:
The purpose of this study is to determine the possible role of the processed peptide of LHRH, LHRH-(1-5), in regulating growth of endometrial cancer cells.
Study Design:
An endometrial cancer cell line, the Ishikawa cell line, was cultured under standard conditions and treated in a dose-dependent manner with 1 of 2 hormones, LHRH and LHRH-(1-5) to determine the ability of these peptides to regulate cellular growth. A tetrazolium-based assay was used to determine the effect these peptides have on cell proliferation. Furthermore, enzyme-linked immunosorbent assay (ELISA)-based assays were used to determine the expression of caspase-3/7 and pERK-1/2. Statistical analyses were conducted using an analysis of variance followed by Fisher LSD as the post-hoc test.
Results:
The results show that LHRH is anti-proliferative whereas LHRH-(1-5) is proliferative on the cells. Furthermore, LHRH-(1-5) decreased caspase-3/7 and pERK1/2 expression.
Conclusion:
This is the first time LHRH-(1-5) is shown to have proliferative effects on cells.
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