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Updated: Jul 17, 2026
Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
DNA liquid-crystalline gel as adsorbent of carcinogenic agent
Toshiaki Dobashi1, Kazuya Furusawa, Erika Kita
1Departments of Biological and Chemical Engineering and Physics, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan. dobashi@bce.gunma-u.ac.jp
Abstract:
DNA liquid crystalline gel (LCG) has been newly prepared by a dialysis of concentrated DNA solutions into concentrated metal cation solutions. The condition for forming DNA LCG is examined by means of the insolubilization reaction. The shrinking ratio and the ratio of the thickness of LCG layer, delta, and the diameter of the dialysis tube, d0, do not depend on d0. The adsorption of one of carcinogenic agents, acridine orange is demonstrated. From the experimental results, the mechanism for forming DNALCG is discussed.
