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A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
Published on: May 1, 2013
Two Lactobacillus strains, isolated from breast milk, differently modulate the immune response
M P Díaz-Ropero1, R Martín, S Sierra
1Immunology and Animal Science Department, Puleva Biotech SA, Granada, Spain.
Journal of Applied Microbiology
|January 24, 2007
Summary
Two Lactobacillus strains from breast milk show distinct immune effects. Lactobacillus fermentum CECT5716 is immunostimulatory, while Lactobacillus salivarius CECT5713 has an anti-inflammatory impact, highlighting strain specificity.
Area of Science:
- Immunology
- Microbiology
- Probiotics
Background:
- Human breast milk contains various beneficial bacteria, including Lactobacillus species.
- These bacteria play a crucial role in infant immune system development.
- Understanding the specific immunomodulatory effects of different Lactobacillus strains is important for their application.
Purpose of the Study:
- To investigate the immunomodulatory capabilities of two Lactobacillus strains: Lactobacillus salivarius CECT5713 and Lactobacillus fermentum CECT5716.
- To compare the effects of these strains on immune responses in vitro and in vivo.
Main Methods:
- In vitro studies using rodent bone-marrow-derived macrophages (BMDM) to assess cytokine production.
- In vivo studies in mice to evaluate immune responses, including cytokine profiles and IgA levels.
Main Results:
- Lactobacillus fermentum CECT5716 induced pro-inflammatory cytokines in BMDM and enhanced Th1 cytokines and fecal IgA in mice.
- Lactobacillus salivarius CECT5713 induced IL-10 in BMDM and spleen cells, demonstrating a more efficient reduction of LPS-induced inflammation.
- Both strains modulated immune responses, but with contrasting effects: immunostimulatory for L. fermentum and anti-inflammatory for L. salivarius.
Conclusions:
- Lactobacillus fermentum CECT5716 exhibits an immunostimulatory effect.
- Lactobacillus salivarius CECT5713 demonstrates an anti-inflammatory effect.
- The study highlights the strain-specific immunomodulatory properties of Lactobacillus isolated from human breast milk.
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