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Published on: February 6, 2018
[Apoptosis and gene FasL expression induced by carbon disulfide in rat sertoli cells]
Jing Deng1, Guo-Yuan Chen, Jia-Jia Ji
1Department of Labor Health & Environment Health, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430030, China.
Objective:
To study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.
Methods:
Sertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.
Results:
Sertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).
Conclusion:
CS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
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Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
Caspases
Apoptosis

