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Identification of a conserved epitope in class I alcohol dehydrogenase isoenzymes using monoclonal antibodies
A Adinolfi1, N Leone, D Swallow
1International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Experimental and Clinical Immunogenetics
|January 1, 1991
Abstract:
Two monoclonal antibodies raised against native horse alcohol dehydrogenase (HADH) bind preferentially to the enzyme attached to solid supports and recognize the denatured and carboxymethylated HADH subunits. Both antibodies cross-react with the human class I isoenzymes but do not recognize the class III ADH isoenzyme. Protease digestion, electrophoresis and HPLC have been used to identify the linear epitope which is contained in the sequence Pro344-Glu357 of the HADH subunit.