Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TrkAIII expression in the thymus.

Antonella Tacconelli1, Antonietta R Farina, Lucia Cappabianca

  • 1Department of Experimental Medicine, University of L'Aquila, Coppito 2, Via Vetoio, 67100 L'Aquila, Italy.

Journal of Neuroimmunology
|January 24, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pro-apoptotic and cell cycle-modulating effects of lobaric and rhizocarpic acids in human leukemic cell lines.

Molecular biology reports·2026
Same author

An orthotopic organoid-based model to study early CD8⁺ T cell dysfunction and immunotherapy response in colorectal cancer.

Oncoimmunology·2026
Same author

Correction: Ciafarone et al. Multi-Strain Probiotic Lysate Attenuates TGF-β1-Induced Intestinal Fibrosis and EMT Modulating Smad, Akt, and WNT/β-Catenin Pathways. <i>Cells</i> 2025, <i>14</i>, 1432.

Cells·2026
Same author

Inflammatory bowel disease and extracellular matrix: when victim becomes double agent.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2026
Same author

Prolyl-isomerase Pin1 drives platinum resistance by regulating Notch3 stability and function in ovarian cancer.

Journal of experimental & clinical cancer research : CR·2026
Same author

Opposite effects of spermidine and GC7 in cell culture are dictated by distinct molecular targets.

The Biochemical journal·2025

The alternative TrkAIII splice variant is found in the thymus, potentially impacting thymocyte development and function. Its expression may offer an alternative to standard nerve growth factor signaling in the thymus.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The TrkA receptor tyrosine kinase plays a role in neuronal development and function.
  • Alternative splicing of TrkA can generate different protein isoforms with potentially distinct functions.
  • The thymus is a primary lymphoid organ crucial for T cell development.

Purpose of the Study:

  • To investigate the expression and localization of the alternative TrkAIII splice variant in murine and human thymuses.
  • To explore the potential role of TrkAIII in thymocyte development and its regulation by environmental factors like hypoxia.
  • To understand how TrkAIII expression might influence nerve growth factor (NGF) signaling within the thymus.

Main Methods:

  • RT-PCR and Western blotting to detect TrkAIII mRNA and protein.

Related Experiment Videos

  • Flow cytometry (FACS) and immunohistochemistry for cellular localization.
  • Cell culture under normoxic and hypoxic conditions.
  • CoCl2 treatment to mimic hypoxia in Jurkat T cells.

    • Main Results:

    • TrkAIII is expressed in murine and human thymuses, predominantly in later developmental stages.
    • TrkAIII protein is localized intracellularly in specific thymocyte subsets and thymic epithelial cells (TECs).
    • TrkAIII expression in thymocytes is influenced by hypoxia, while TEC expression is less affected.
    • TrkAIII expression is associated with thymocyte proliferation and may impede NGF/TrkA signaling.

    Conclusions:

    • TrkAIII is a significant splice variant in the thymus, particularly relevant for CD4+/CD8+ thymocytes and TECs.
    • Hypoxia may induce TrkAIII splicing in thymocytes, suggesting an adaptation to the thymic microenvironment.
    • Intracellular TrkAIII may act as an alternative or impediment to canonical NGF/TrkA signaling, impacting thymic function.