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Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

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Related Experiment Video

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Dissection of Oenocytes from Adult Drosophila melanogaster

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Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional

Georgios Banilas¹, Alkis Nikiforiadis, Ifigenia Makariti

¹Laboratory of Molecular Biology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.

Tree Physiology

|January 24, 2007

Summary

Olive plants utilize omega-3 fatty acid desaturase (OeFAD3) for alpha-linolenic acid (alpha-LeA) production, crucial for female reproductive development, not male fertility.

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Area of Science:

Plant Physiology
Molecular Biology
Biochemistry

Background:

Alpha-linolenic acid (alpha-LeA) abundance relative to linoleic acid impacts plant development.
Omega-3 fatty acid desaturases (FADs) catalyze linoleic acid to alpha-LeA conversion.
FADs are located in plastids or the endoplasmic reticulum (ER).

Purpose of the Study:

Isolate and characterize an ER-type omega-3 FAD gene (OeFAD3) from olive (Olea europaea L.).
Investigate the role of OeFAD3 in olive fruit development and oil biosynthesis.
Determine the function of alpha-LeA in olive reproductive development.

Main Methods:

Gene isolation and characterization of OeFAD3 from olive.
Analysis of OeFAD3 expression patterns in various olive tissues and developmental stages.
Comparison of OeFAD3 regulation with plastidial FADs and studies on JA-deficient mutants.

Main Results:

OeFAD3 expression contributes minimally to olive oil biosynthesis.
OeFAD3 is preferentially expressed in proliferating tissues, supporting membrane biogenesis.
OeFAD3 upregulation in pistil tissues suggests alpha-LeA's role in female gametophyte development.

Conclusions:

OeFAD3 plays a limited role in olive oil modification but is vital for reproductive development.
Alpha-linolenic acid is essential for female gametophyte development in olive.
Findings align with studies on jasmonic acid-deficient mutants in other species.