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Published on: July 22, 2011
Lactobacillus paracasei subsp. paracasei B21060 suppresses human T-cell proliferation
Ilaria Peluso1, Daniele Fina, Roberta Caruso
1Dipartimento di Medicina Interna, Università Tor Vergata, Via Montpellier 1, 00133 Rome, Italy.
Infection and Immunity
|January 24, 2007
Summary
Lactobacillus paracasei subsp. paracasei B21060 inhibits T-cell growth, offering a potential mechanism for probiotic intervention in inflammatory diseases. This specific probiotic strain impacts T-cell proliferation without altering key immune markers.
Area of Science:
- Immunology
- Microbiology
- Gastroenterology
Background:
- Probiotics show promise in managing T-cell-mediated inflammatory diseases.
- The precise molecular mechanisms underlying probiotic immunomodulation are not fully understood.
- T cells possess receptors for bacterial components, suggesting direct interaction is possible.
Purpose of the Study:
- To investigate the direct effects of specific Lactobacillus strains on human T-cell function.
- To determine if Lactobacillus paracasei subsp. paracasei B21060 modulates T-cell proliferation, survival, and cytokine production.
Main Methods:
- Isolation of CD4(+) T cells from blood, intestinal lamina propria (LP), and Peyer's patches.
- Activation of cells with anti-CD3/CD2/CD28 in the presence or absence of specific Lactobacillus strains.
- Evaluation of cell proliferation, death, Foxp3 expression, intracellular pH, and cytokine profiles via flow cytometry.
Main Results:
- Lactobacillus paracasei subsp. paracasei B21060 significantly inhibited CD4(+) T-cell proliferation from blood.
- This inhibition was linked to decreased MCT-1 expression and reduced intracellular pH, independent of cell survival or major cytokine changes.
- The probiotic also suppressed the growth of Peyer's patch mononuclear cells and lymphocytes from normal individuals and IBD patients.
Conclusions:
- Lactobacillus paracasei subsp. paracasei B21060 possesses the ability to block T-cell proliferation.
- This finding suggests a novel mechanism by which probiotics may regulate T-cell-driven immune responses in inflammatory conditions.
