Related Experiment Videos
[Epitope analysis of CD68 antigens]
D Ulsijchutagijn1, H Kupper, W Eichler
1Sektion Biowissenschaften, Universität Leipzig, Germany.
Allergie Und Immunologie
|January 1, 1991
Summary
Three new monoclonal antibodies (MABs) identify distinct CD68 antigen epitopes. One MAB detects a stable epitope in fixed tissues, increasing known CD68 epitopes to at least seven.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Context:
- CD68 is a key protein in macrophage biology and immune responses.
- Characterizing CD68 epitopes is crucial for understanding its function and developing diagnostic tools.
Purpose:
- To generate and characterize novel monoclonal antibodies (MABs) targeting the CD68 antigen.
- To define new epitopes on CD68 using immunohistochemical blocking experiments.
Summary:
- Three new mouse IgM monoclonal antibodies (MABs), BL-M68/1-3, were generated against different CD68 antigen epitopes.
- MAB BL-M68/3 recognizes an epitope stable in formalin-fixed paraffin-embedded tissues.
- Blocking experiments with these and other MABs defined two new CD68 epitopes, increasing the known count to at least seven.
Impact:
- Expands the repertoire of tools for CD68 research.
- Facilitates more precise immunohistochemical analysis of CD68 in various biological and pathological contexts.
- Contributes to a deeper understanding of CD68 epitope diversity and function.