Controlling multiple fluorescent signal output in cyclic peptide-based supramolecular systems

Area of Science:

• Supramolecular Chemistry
• Biophysical Chemistry
• Chemical Biology

Background:

• Self-assembling peptides offer versatile platforms for constructing complex molecular architectures.
• Controlled fluorescence output is crucial for monitoring molecular interactions in real-time.
• Förster Resonance Energy Transfer (FRET) and excimer formation are powerful tools for studying molecular proximity and interactions.

Purpose of the Study:

• To develop a multicomponent equilibrium network utilizing self-assembling alpha,gamma-cyclic peptides.
• To achieve controlled fluorescence output for studying complex interaction networks.
• To explore the combined use of excimer and FRET effects for network analysis.

Main Methods:

• Synthesis and characterization of alpha,gamma-cyclic peptides.
• Investigation of peptide self-assembly and dimer formation (homo- and heterodimers).
• Utilizing fluorescence spectroscopy to monitor excimer formation and FRET between labeled peptides.
• Analysis of multicomponent equilibrium and network formation.

Main Results:

• Demonstrated successful construction of a multicomponent equilibrium network based on self-assembling alpha,gamma-cyclic peptides.
• Achieved controlled fluorescence output modulated by peptide self-assembly and dimer formation.
• Showcased the synergistic application of excimer and FRET effects for studying complex interactions.
• Reported the first investigation of the Dapoxyl/pyrene FRET pair in this context.

Conclusions:

• The developed peptide-based network provides a robust platform for studying complex molecular interactions.
• The combined use of excimer and FRET effects offers enhanced capabilities for analyzing intricate supramolecular systems.
• This work establishes a new methodology for fluorescence-based interrogation of complex chemical and biological networks.