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Updated: Jul 17, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Novel coumarin-based fluorescent pH indicators, probes and membranes covering a broad pH range
Anna S Vasylevska1, Alexander A Karasyov, Sergey M Borisov
1Chemo- and Biosensors, Institute of Analytical Chemistry, University of Regensburg, 93040, Regensburg, Germany. anna.vasilevskaya@chemie.uni-regensburg.de
Abstract:
A new family of coumarin-based pH indicators was synthesized. They are sensitive to pH in either weakly acidic or weakly basic solution. The indicators possess moderate to high brightness, excellent photostability and compatibility with light-emitting diodes. The indicators were covalently immobilized on the surface of amino-modified polymer microbeads which in turn were incorporated into a hydrogel matrix to obtain novel pH-sensitive materials. When a mixture of two different microbeads is used, the membranes are capable of optical pH sensing over a very wide range comparable to the dynamic range of the glass electrode (pH 1-11).
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