Related Experiment Video
Updated: Jul 17, 2026
15:25
Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
Published on: March 16, 2010
Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences
1Department of Horticulture, New Mexico State University, Las Cruces, New Mexico 88003.
Genetics
|April 1, 1986
Summary
Researchers developed a comprehensive tomato linkage map using isozyme and complementary DNA (cDNA) markers. This molecular map covers 92% of the tomato genome, enabling advanced genetic studies and breeding innovations.
Area of Science:
- Plant genetics
- Molecular biology
- Genomics
Background:
- Developing high-density genetic maps is crucial for understanding plant genomes.
- Isozyme and complementary DNA (cDNA) markers have been instrumental in plant genetic mapping.
Purpose of the Study:
- To construct a high-resolution linkage map in tomato (Lycopersicon esculentum).
- To integrate isozyme, cDNA, and DNA markers for comprehensive genomic coverage.
Main Methods:
- Utilized interspecific backcross and F(2) populations from Lycopersicon esculentum x L. pennellii crosses.
- Employed restriction fragment length polymorphisms (RFLPs) for cDNA marker analysis via Southern blotting.
- Analyzed 57 unique cDNA clones and integrated them with existing isozyme and DNA markers.
Main Results:
- Developed a linkage map comprising 112 loci (isozyme, cDNA, and DNA markers).
- The integrated map covers an estimated 92% of the tomato genome.
- Most cDNA markers identified single loci, dispersed throughout the genome, with many showing genetic independence.
Conclusions:
- The constructed molecular map provides extensive genomic coverage in tomato.
- This map facilitates advanced genetic analyses and breeding strategies previously unattainable.
- The integration of diverse marker types enhances the utility of the tomato genome map.
