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Related Experiment Videos

DNA from Maize with and without B Chromosomes: A Comparative Study.

M D Chilton1, B J McCarthy

  • 1Departments of Microbiology, University of Washington, Seattle, Washington 98195.

Genetics
|August 1, 1973
PubMed
Summary

Maize B chromosomes share DNA sequences with A chromosomes. DNA competition and renaturation studies show their genetic material is nearly identical, suggesting close evolutionary relation.

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Maize (Zea mays) possesses standard A chromosomes and accessory B chromosomes.
  • The genetic makeup and relationship of B chromosome DNA to A chromosome DNA remain incompletely understood.

Purpose of the Study:

  • To investigate the molecular relationship between the DNA of maize B chromosomes and A chromosomes.
  • To determine if B chromosome DNA contains unique sequences absent from A chromosomes.

Main Methods:

  • Buoyant density centrifugation of DNA in cesium chloride (CsCl) gradients.
  • DNA renaturation kinetics analysis under various stringency conditions.
  • DNA competition hybridization studies.
  • Melting profile analysis of homologous and heterologous DNA duplexes.

Main Results:

  • DNA from 5B and 0B maize seedlings exhibited identical buoyant density distributions.
  • Renaturation kinetics were indistinguishable between 5B and 0B DNA.
  • DNA competition assays did not reveal unique redundant sequences in 5B DNA absent in 0B DNA.
  • Melting profiles of DNA duplexes formed between 5B and 0B DNA were virtually identical.

Conclusions:

  • The DNA of maize B chromosomes is highly similar, if not identical, to that of A chromosomes.
  • B chromosomes do not appear to contain significantly different or unique DNA components compared to A chromosomes.
  • This suggests a close genetic and evolutionary relationship between maize A and B chromosomes.

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