Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A mutant killer plasmid whose replication depends on a chromosomal "superkiller" mutation.

A Toh-E1, R B Wickner

  • 1Laboratory of Biochemical Pharmacology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryand 20014.

Genetics
|April 1, 1979
PubMed
Summary

Yeast killer plasmid variants ([KIL-sd]) require specific chromosomal mutations (ski) for replication. These ski-dependent plasmids are lost in normal yeast strains, revealing a novel form of mutagenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yeast and Fungal Prions: Amyloid-Handling Systems, Amyloid Structure, and Prion Biology.

Advances in genetics·2016
Same author

Prions of yeast from cytoplasmic genes to heritable amyloidosis.

Methods in molecular medicine·2011
Same author

Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically encodable tandem ubiquitin.

Methods in enzymology·2005
Same author

Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity.

Methods in enzymology·2005
Same author

Prions of yeast are genes made of protein: amyloids and enzymes.

Cold Spring Harbor symposia on quantitative biology·2005
Same author

Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae.

Genes & genetic systems·2002

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Virology

Background:

  • Yeast strains with the killer plasmid (M dsRNA) produce a toxin lethal to non-carrier strains.
  • Chromosomal mutations in ski genes (ski1-ski4) enhance toxin production.

Purpose of the Study:

  • To characterize a novel killer plasmid mutation, [KIL-sd], dependent on chromosomal ski mutations for replication.
  • To investigate the interaction between killer plasmid variants and yeast chromosomal mutations.

Main Methods:

  • Introduction of wild-type killer plasmid ([KIL-k]) into ski mutant yeast strains.
  • Observation of killer plasmid stability and replication in different genetic backgrounds.
  • Analysis of killer plasmid conversion to the ski-dependent form ([KIL-sd]).

Related Experiment Videos

Main Results:

  • A ski-dependent killer plasmid ([KIL-sd]) was identified, requiring ski gene mutations for its maintenance.
  • [KIL-sd] plasmids are lost in wild-type (SKI+) yeast strains.
  • The wild-type killer plasmid ([KIL-k]) can convert to [KIL-sd] in specific ski mutant strains (e.g., ski2-2), suggesting mutagenesis or selective replication.
  • The ski2-3 mutation supports [KIL-sd] maintenance but does not induce conversion from [KIL-k].

Conclusions:

  • The [KIL-sd] plasmid lacks essential replication elements for wild-type yeast cells.
  • The ski mutations play a critical role in the replication and maintenance of specific killer plasmid variants.
  • The conversion of [KIL-k] to [KIL-sd] in ski mutants represents a unique phenomenon in yeast-plasmid interactions.