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Related Experiment Video

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Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
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An unstable nuclear gene in phycomyces.

M Delbrück1, T Ootaki

  • 1Division of Biology, California Institute of Technology, Pasadena, California 91125.

Genetics
|May 1, 1979
PubMed
Summary

Genetic instability in Phycomyces, linked to the dar gene, affects riboflavin uptake. Mutants show resistance to deaza-riboflavin, indicating a crucial role for this gene in nutrient transport and genetic stability.

Area of Science:

  • * Genetics
  • * Molecular Biology
  • * Mycology

Background:

  • * Phycomyces exhibits genetic instability linked to a specific nuclear gene, designated dar.
  • * Wild-type strains efficiently uptake riboflavin and its toxic analog, deaza-riboflavin, even at low concentrations.
  • * Mutants display impaired riboflavin uptake and resistance to deaza-riboflavin, suggesting a role in transport mechanisms.

Purpose of the Study:

  • * To characterize the genetic instability associated with the dar gene in Phycomyces.
  • * To investigate the role of the dar gene in riboflavin and deaza-riboflavin transport.
  • * To determine the mutation rates and allelic interactions of dar mutants.

Main Methods:

  • * Genetic analysis of Phycomyces strains.

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  • * Heterokaryon complementation tests.
  • * Measurement of riboflavin and deaza-riboflavin uptake.
  • * Estimation of forward and reverse mutation rates.

    • Main Results:

    • * A single nuclear gene, dar, is identified as responsible for the observed genetic instability.
    • * dar mutants are unable to uptake riboflavin and exhibit resistance to deaza-riboflavin.
    • * Forward and reverse mutation rates were estimated at 4 x 10(-5) and 2 x 10(-3) per nuclear division, respectively.
    • * Mutants showed no complementation in heterokaryons and were largely recessive.
    • * Spore phenotype was influenced by the allele ratio within the sporangium.

    Conclusions:

    • * The dar gene plays a critical role in riboflavin uptake and deaza-riboflavin resistance in Phycomyces.
    • * The dar gene is essential for maintaining genetic stability.
    • * The recessive nature and non-autonomous phenotype suggest complex genetic interactions and regulation.