Related Experiment Video
Updated: Jul 17, 2026
05:53
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
The intergradation, genetic interchangeability and interpretation of gene conversion spectrum types
Genetics
|May 1, 1979
Summary
Gene conversion in Ascobolus immersus shows variable results based on genetic background, challenging simple molecular interpretations. Further factors like cryptic mutations likely influence these complex gene conversion properties.
Area of Science:
- * Molecular and population genetics
- * Eukaryotic microbial genetics
Background:
- * Gene conversion is a key process in genetic recombination.
- * Understanding gene conversion mechanisms is crucial for genetic studies.
Purpose of the Study:
- * To investigate gene conversion properties of induced and spontaneous mutations in Ascobolus immersus.
- * To analyze the influence of genetic background on gene conversion outcomes.
Main Methods:
- * Studied 29 induced and 9 spontaneous mutations in Ascobolus immersus.
- * Performed crosses between mutants and three types of wild-type strains.
- * Analyzed post-meiotic segregation and conversion frequencies.
Main Results:
- * Mutants exhibited varied conversion results across different crosses.
- * Conversion spectrum types were not distinct and showed intergradation.
- * Genetic differences between strains led to interchangeability of spectrum types for the same mutation.
Conclusions:
- * Previous interpretations of spectrum types based on molecular mutation types are inadequate.
- * Heterozygous cryptic mutations or conversion control genes likely influence gene conversion.
- * Asymmetric hybrid DNA formation may cause discrepancies between correction and observed conversion properties.
Related Concept Videos
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
02:18Types of Genetic Transfer Between Organisms
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
02:18Types of Genetic Transfer Between Organisms
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
02:13Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...