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Normal psoriatic epidermis expression of hyperproliferation-associated keratins
Archives of Dermatological Research
|January 1, 1991
Summary
Psoriatic skin shows altered keratin patterns, with specific keratins (K6, K16) appearing in uninvolved areas and changes in K1, K5, K10, and K14 levels. This keratin alteration may explain the Köbner effect in psoriasis.
Area of Science:
- Dermatology
- Molecular Biology
- Biochemistry
Background:
- Psoriasis is a chronic inflammatory skin disease characterized by epidermal hyperplasia.
- Keratin intermediate filaments are crucial structural components of keratinocytes and their expression patterns are altered in various skin diseases.
Purpose of the Study:
- To investigate the differential expression of keratins in lesional, marginal, and uninvolved psoriatic epidermis.
- To correlate keratin expression changes with the altered differentiation patterns observed in psoriatic skin.
Main Methods:
- Analysis of keratin expression using one- and two-dimensional gel electrophoresis and immunoblotting.
- Laser-densitometric evaluation of protein levels from gel electrophoresis.
- Comparison of keratin profiles in psoriatic epidermis versus normal epidermis.
Main Results:
- Identification of keratins K1, K5, K6, K10, K14, and K16 in lesional psoriatic epidermis.
- Keratins K6 and K16 were present in uninvolved psoriatic skin but absent in normal epidermis.
- Downregulation of K1 and K10, upregulation of K6 and K16, decreased K5, and increased K14 in lesional psoriatic epidermis compared to normal skin.
Conclusions:
- Psoriatic epidermis exhibits significant alterations in keratin expression, affecting both lesional and uninvolved skin.
- Suprabasal keratinocytes in uninvolved psoriatic skin show an altered differentiation pattern.
- These keratin expression changes are potentially key to understanding the Köbner phenomenon in psoriasis.