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Updated: Jul 17, 2026

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Published on: December 5, 2016
A method for complementation analysis of nuclear and chloroplast mutants of photosynthesis in chlamydomonas
P Bennoun1, A Masson, M Delosme
1Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris France.
Abstract:
The photosynthetic properties of young zygotes of Chlamydomonas reinhardi were analyzed. In heterozygotes for two nuclear or two chloroplast mutations affecting photosynthesis, recovery of photosynthetic activity was observed that is most likely the result of intergenic complementation.--We observed that chloramphenicol inhibited the recovery of activity in double heterozygotes for mutants lacking at least one thylakoid polypeptide of chloroplast origin, while it had not effect on wild-type homozygotes. This indicates that the recovery of activity in double heterozygotes could result from the repair of existing thylakoid membranes by de novo synthesis of the missing polypeptides.
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