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Related Experiment Video

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Lateral Root Inducible System in Arabidopsis and Maize

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Controlling-element events at the shrunken locus in maize.

B Burr¹, F A Burr

¹Department of Biology, Brookhaven National Laboratory, Upton, NY 11973.

|May 1, 1981

Summary

Researchers studied controlling element Ds insertions in maize Shrunken genes. They found large DNA insertions, over 20 kilobase pairs, providing a basis for molecular characterization of these maize controlling elements.

Area of Science:

Molecular Biology
Genetics
Plant Science

Background:

Controlling elements, like Ds, are mobile genetic sequences in maize.
Understanding their insertion mechanisms is crucial for gene regulation studies.

Purpose of the Study:

To investigate the molecular nature of Dissociation (Ds) element insertions at the Shrunken locus in maize.
To provide a foundation for isolating and characterizing maize controlling elements at the molecular level.

Main Methods:

Preparation of a complementary DNA (cDNA) probe specific to the Shrunken locus mRNA.
Utilizing maize lines with specific chromosome 9 short arm derivatives.
Detection of nucleic acid modifications using the prepared cDNA probe.

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Main Results:

The Shrunken locus in maize exhibits modifications at the nucleic acid level due to Ds insertions.
Detected changes indicate large DNA insertions, with one potentially exceeding 20 kilobase pairs.
The cDNA probe successfully identified unique sequences within maize DNA, confirming locus specificity.

Conclusions:

Ds element insertions at the Shrunken locus result in significant genomic alterations.
These findings facilitate the molecular isolation and characterization of maize controlling elements.
The study offers insights into the impact of mobile genetic elements on plant genomes.