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Identification of phosphotyrosine residues during protein sequence analysis
C W Turck1, J Herrmann, J A Escobedo
1Howard Hughes Medical Institute, University of California, San Francisco.
Peptide Research
|January 1, 1991
Abstract:
Synthetic tyrosine-phosphorylated peptides were subjected to protein sequence analysis using a gas-phase sequencer and on-line phenylthiohydantoin (PTH) amino acid analysis. Our data show that phosphotyrosine is stable to the gas-phase sequencing chemistry and can be detected as its PTH-derivative during routine sequence analysis without the need of prior tyrosine radiolabeling.