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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR

Kirstine Klitgaard1, Tim K Jensen, Øystein Angen

  • 1National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.

Journal of Microbiological Methods
|January 26, 2007
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...