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5-Hydroxytryptamine releasing activity of the supernatants from cultured mouse spleen cells
1Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Archivum Immunologiae Et Therapiae Experimentalis
|January 1, 1991
Abstract:
Mouse spleen cells have been shown to produce a histamine releasing factor (HRF) after stimulation with mitogen or specific antigen in vitro. The supernatants from the cultures of mouse spleen cells released not only histamine but also 5-hydroxytryptamine (5-HT) from homologous mast cells in a dose-dependent manner. The time-course of this release was similar to that observed in antigen or anti-IgE reaction. Heavy water (D2O) enhanced supernatant-induced mediator release.