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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

[An interferon inhibitor in regulating the activity of human natural killer cells]

S B Cheknev, Narovlianskiĭ, A M Amchenkova

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |October 1, 1991
    PubMed

    The action of mouse serum interferon--alpha/beta (IFN) at the dose of 100 U/ml, of its inhibitor (I) at the dose of 8 U/ml as well as of their combination with the above doses on sensitivities of mouse target cells (TC) of sensitive to IFN line L 929 and resistant to the one line MCB in natural cytotoxic reaction was studied. Cytotoxic activity of human natural killer cells was detected in 14 hrs cytotoxic test using 3H-uridine for labelling of TC. IFN, I, and IFN+I were added to cell cultures for 24 hrs at 37 degrees C with following removing of preparations. It has been shown that I abolished protective effect of IFN on TC L 929 whereas the one possessed the protective action on TC MCB in natural cytotoxic reaction. These data confirmed a suggestion about immunoregulatory properties of I which displayed in abolition or realization of protective effect on TC in natural cytotoxic reaction in dependence on initial sensitivity of TC to antiviral IFN action.

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