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Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Nitric oxide in pulp cell growth, differentiation, and mineralization
R Yasuhara1, T Suzawa, Y Miyamoto
1Department of Biochemistry, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Journal of Dental Research
|January 26, 2007
Summary
Nitric oxide (NO) plays a role in pulpitis following dental procedures. This study found NO influences pulp cell growth, mineralization, and apoptosis, suggesting its involvement in pulp tissue changes.
Area of Science:
- Biomedical Science
- Dental Research
- Cell Biology
Background:
- Dental preparation can lead to pulp inflammation, edema, and necrosis.
- The role of nitric oxide (NO) in these pulp pathophysiological changes is not fully understood.
Purpose of the Study:
- To investigate the involvement of inducible nitric oxide synthase (iNOS) in murine pulp after dental preparation.
- To examine the effects of NO on pulp cell proliferation, mineralization, and apoptosis in vitro.
Main Methods:
- Examined mRNA and protein expression of iNOS in murine pulp post-dental preparation.
- Utilized an NO donor (NOC-18) to study its effects on cultured pulp cells.
Main Results:
- iNOS mRNA and protein were expressed in pulp after preparation.
- NO donor suppressed pulp cell proliferation but promoted mineralization and induced apoptosis under specific conditions.
Conclusions:
- Nitric oxide (NO) appears to regulate pulp cell growth, mineralization, and apoptosis.
- NO may be a key mediator in the pulp's response to dental preparation.
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