CNS active principles from Alangium plantanifolium

Area of Science:

• Phytochemistry
• Neuropharmacology
• Natural Products Chemistry

Background:

• The root bark of Alangium plantanifolium is a source of bioactive compounds.
• Central nervous system (CNS) receptor binding is a key area for drug discovery.

Purpose of the Study:

• To identify compounds from Alangium plantanifolium root bark extract with CNS receptor binding activity.
• To investigate the binding affinities of isolated compounds to specific CNS receptors.

Main Methods:

• Extraction of Alangium plantanifolium root bark with 70% ethanol.
• Isolation and identification of compounds using Mass spectrometry, 1H-NMR, and 13C-NMR.
• Assessment of receptor binding activities and IC50 values for isolated compounds.

Main Results:

• Five compounds were identified: beta-sitosterol, stigmasterol, sitosterol 3-O-beta-D-glucopyranoside, a disaccharide, and a potentially new glucoside (5beta,6beta-dihydroxycyclohex-2-en-1-ol 1-beta-glucoside).
• Stigmasterol and the novel glucoside exhibited binding to muscarinic receptors with IC50 values of 8.52 microM and 6.74 microM, respectively.
• Beta-sitosterol, sitosterol 3-O-beta-D-glucopyranoside, and the novel glucoside influenced the binding of other compounds to CNS receptors.

Conclusions:

• Alangium plantanifolium root bark contains compounds with significant CNS receptor binding properties.
• Stigmasterol and the novel glucoside are promising candidates for further investigation in neuropharmacology.
• The identified compounds may modulate CNS receptor activity through various mechanisms.