Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immobilization of enzymes on PTFE surfaces.

Biotechnology and bioengineering·2001
Same author

Cysteine sulfoxides and alliinase activity of some Allium species.

Journal of agricultural and food chemistry·2000
Same author

Immobilization of alliinase on porous aluminum oxide.

Biotechnology and bioengineering·2000
Same author

Stabilization and pharmaceutical use of alliinase.

Die Pharmazie·1999
Same author

Quality of herbal remedies from Allium sativum: differences between alliinase from garlic powder and fresh garlic.

Planta medica·1999
Same author

Sulfoquinovosyl diacylglycerols from the alga Heterosigma carterae.

Lipids·1997
Same journal

Ginsenoside Rd ameliorates glucose metabolism by regulating GLP-1/ MAPK/ NF-κB signaling pathway in db/db mice.

Planta medica·2026
Same journal

Therapeutic Potential of Limonium brasiliense Extracts in Helicobacter pylori-Associated Gastric Pathologies.

Planta medica·2026
Same journal

Phytochemical study and antibacterial activity of Cedrus atlantica aerial part apolar extract.

Planta medica·2026
Same journal

Selection of promising candidates for herbal veterinary medicinal products: A strategic approach.

Planta medica·2026
Same journal

Life and Work of Ernst Reinhard (1926 - 2005).

Planta medica·2026
Same journal

Glechoma hederacea: A Comprehensive Insight into the Botanical Specification, Traditional Uses, Phytochemistry, Nutritional Value, Pharmacology, and Non-medicinal Applications.

Planta medica·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
10:09

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Published on: March 15, 2017

A high-throughput method for the quantitative determination of alliin.

M Keusgen1

  • 1Institut für Pharmazeutische Biologie, Universität Bonn, Germany. M.KEUSGEN@uni-bonn.de

Planta Medica
|January 27, 2007
PubMed
Summary
This summary is machine-generated.

A new analytical method quantifies alliin in garlic (Allium sativum L.) preparations. This high-throughput technique uses immobilized alliinase and flow injection analysis, offering a faster alternative to HPLC for quality control.

More Related Videos

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
09:10

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

Published on: June 12, 2026

Related Experiment Videos

Last Updated: Jun 24, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
10:09

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Published on: March 15, 2017

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
09:10

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

Published on: June 12, 2026

Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Food Science

Background:

  • Garlic (Allium sativum L.) quality relies on alliin content in various preparations.
  • Accurate alliin quantification is crucial from raw material to final products.
  • Conventional HPLC methods can be time-consuming for high-throughput analysis.

Purpose of the Study:

  • To develop a novel, rapid analytical method for alliin quantification in garlic.
  • To provide comprehensive documentation of alliin concentration throughout garlic product processing.
  • To establish a high-throughput alternative to traditional HPLC methods.

Main Methods:

  • Utilized immobilized alliinase (EC 4.4.1.4) coupled with a two-channel flow injection analyser (FIA).
  • Integrated an ammonia detecting device for sensitive alliin measurement.
  • Avoided pre-column derivatization and chromatographic separation for increased speed.

Main Results:

  • Demonstrated high specificity for alliin using the developed method.
  • Successfully analyzed alliin concentrations in diverse garlic samples (powders, dry extracts, preparations).
  • Achieved good correlation between new method results and conventional HPLC data.

Conclusions:

  • The novel immobilized alliinase-FIA method offers a specific and efficient way to quantify alliin.
  • This technique facilitates high-throughput analysis, essential for garlic quality control.
  • The method provides reliable results comparable to HPLC, supporting comprehensive documentation.