Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytokine and chemokine profiles linked to early severity of scrub typhus: multicenter validation of soluble PD-L1.

Journal of clinical microbiology·2026
Same author

Host traits shape flea infestation patterns in small mammals: a case study of <i>Spermophilus undulatus</i> and associated flea species in northern Xinjiang, China.

Frontiers in veterinary science·2026
Same author

Methadone maintenance therapy alters the expression of PLK1 and IFIH1 in patients with heroin addiction: a case-control study.

Psychopharmacology·2026
Same author

Correction: Identification of serum MicroRNAs associated with hepatic immunoinflammatory injury in chronic hepatitis B: implications for non-invasive diagnosis.

Frontiers in immunology·2026
Same author

Identification of serum MicroRNAs associated with hepatic immunoinflammatory injury in chronic hepatitis B: implications for non-invasive diagnosis.

Frontiers in immunology·2025
Same author

PFDN6L Gene Predicts Good Prognosis Associated with Its Inhibition of the Stem-Ness Properties in Hepatocellular Carcinoma.

Oncology research·2025

Related Experiment Video

Updated: Jul 17, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Microfluidic library screening for mapping antibody epitopes.

Paul H Bessette1, Xiaoyuan Hu, Hyongsok T Soh

  • 1Department of Chemical Engineering, and Institute for Collaborative Biotechnologies, University of California, Santa Barbara, California 93106, USA.

Analytical Chemistry
|January 27, 2007
PubMed
Summary

Researchers developed a disposable microfluidic device to screen large molecular libraries. This innovation simplifies reagent generation and enables advanced bioanalytical systems for diagnostics.

More Related Videos

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Related Experiment Videos

Last Updated: Jul 17, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Disposable microfluidic devices offer potential for automated reagent generation and integrated bioanalytical systems.
  • Screening molecular libraries is crucial for drug discovery and diagnostics.

Purpose of the Study:

  • To map antibody epitopes using a disposable microfluidic device.
  • To screen a large combinatorial peptide library displayed on bacterial cells.

Main Methods:

  • Utilized a two-stage, continuous-flow microfluidic sorter for on-chip library screening.
  • Employed dielectrophoretic funneling to capture antibody-binding target cells on microspheres.
  • Screened a 5 x 10^8 member bacterial-displayed peptide library.

Main Results:

  • Successfully mapped antibody epitopes using the microfluidic device.
  • Achieved on-chip screening of a large-scale combinatorial peptide library.
  • Generated antibody fingerprints comparable to commercial cell sorting instrumentation.

Conclusions:

  • Disposable microfluidic devices are effective for high-throughput screening of molecular libraries.
  • This technology can advance the development of integrated bioanalytical systems for diagnostics.
  • The microfluidic approach offers a simplified and automated method for epitope mapping.