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Big endothelin in plasma and amniotic fluid.

J Chou¹, Y N Wang, D Chang

¹Peninsula Laboratories, Incorporated, Belmont, California 94002.

Journal of Cardiovascular Pharmacology

|January 1, 1991

Summary

Endothelin-1 (ET-1) and big endothelin (big ET) are found in amniotic fluid (AF). Concentrations of both ET-1 and big ET significantly increase in AF from mid-trimester to full-term pregnancy.

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Area of Science:

Reproductive Biology
Endocrinology
Biochemistry

Background:

Endothelin-1 (ET-1) is present in both circulation and amniotic fluid (AF).
Understanding ET-1 and its precursor, big endothelin (big ET), in AF is crucial for pregnancy research.

Purpose of the Study:

To quantify ET-1 and big ET concentrations in plasma and AF.
To identify the molecular forms of endothelin in human AF using advanced techniques.

Main Methods:

Radioimmunoassay (RIA) was used to measure ET-1 and big ET levels.
High-performance liquid chromatography (HPLC) coupled with RIA characterized endothelin molecular forms in AF.
C18 cartridges were used for sample extraction from plasma and AF.

Main Results:

Both ET-1 and big ET were detected in plasma and AF.
Significant increases in AF concentrations of big ET and ET-1 were observed from mid-trimester to full-term pregnancy (p < 0.01).
HPLC analysis revealed ET-1 as a major form in AF, while big ET existed as two components, including endothelin fragment [22-38] (EF).

Conclusions:

Three molecular forms of endothelin—ET-1, big ET, and EF—are present in amniotic fluid.
Concentrations of ET-1 and big ET are elevated in the AF of women at full-term pregnancy compared to mid-trimester.