A fast, sensitive HPLC method for the determination of esterase activity on alpha-tocopheryl acetate
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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
El Hassan Ajandouz1, Stéphane Castan, Stefan Jakob
1Institut Méditerranéen de Recherche en Nutrition, INRA-UMR 1111, Faculté des Sciences et Techniques de Saint-Jérôme, Université Paul Cézanne-Aix Marseille III, France. el.hassan.ajandouz@univ-cezanne.fr
Abstract:
Although data on the behavior of tocopheryl acetate (Tac) in the gut is rare, some studies show that this ester is hydrolyzed in the intestine and the released tocopherol (Tol) is absorbed. An HPLC method is published for the simultaneous analysis of Tol and Tac in mixed micelles. This study shows how this method is used for the measurement of esterase activity on Tac using commercial cholesterol esterase. The rate of hydrolysis is determined in less than 20 min on the basis of both Tac disappearance and Tol appearance. The present method is useful for investigating esterase activities on Tac in model systems that mimick the absorption environment or in any biological or nonbiological medium.
