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Published on: November 23, 2016
A fast, sensitive HPLC method for the determination of esterase activity on alpha-tocopheryl acetate
El Hassan Ajandouz1, Stéphane Castan, Stefan Jakob
1Institut Méditerranéen de Recherche en Nutrition, INRA-UMR 1111, Faculté des Sciences et Techniques de Saint-Jérôme, Université Paul Cézanne-Aix Marseille III, France. el.hassan.ajandouz@univ-cezanne.fr
Journal of Chromatographic Science
|January 27, 2007
Summary
This study quantifies esterase activity on tocopheryl acetate (Tac) using a rapid HPLC method. The developed assay measures both Tac breakdown and tocopherol (Tol) formation, aiding research into nutrient absorption.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Nutritional Science
Background:
- Limited data exists on tocopheryl acetate (Tac) behavior in the gastrointestinal tract.
- Existing research suggests intestinal hydrolysis of Tac to absorbable tocopherol (Tol).
Purpose of the Study:
- To adapt a published HPLC method for simultaneous analysis of Tol and Tac in mixed micelles.
- To quantify esterase activity on Tac using commercial cholesterol esterase.
- To establish a rapid method for assessing Tac hydrolysis in various media.
Main Methods:
- Utilized High-Performance Liquid Chromatography (HPLC) for simultaneous quantification of tocopherol (Tol) and tocopheryl acetate (Tac).
- Employed commercial cholesterol esterase to determine esterase activity on Tac in a model system.
- Measured hydrolysis rates by monitoring both Tac disappearance and Tol appearance.
Main Results:
- The hydrolysis rate of Tac was determined in under 20 minutes.
- The method successfully measured esterase activity by tracking Tac breakdown and Tol formation.
- The assay proved effective in a model system mimicking intestinal absorption.
Conclusions:
- A rapid and efficient HPLC-based method was developed for measuring esterase activity on tocopheryl acetate (Tac).
- This method facilitates the investigation of Tac hydrolysis in diverse biological and non-biological systems.
- The assay is valuable for understanding nutrient absorption and esterase function.
