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Perfluorocarbon in microcirculation during ischemia reperfusion.

Pedro Cabrales1, Amy G Tsai, Marcos Intaglietta

  • 1La Jolla Bioengineering Institute, La Jolla, CA.; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Journal of the American College of Surgeons
|January 27, 2007
PubMed
Summary

Administering perfluorocarbon (PFC) emulsions after ischemia reduces leukocyte activation and improves blood flow, mitigating reperfusion injury. However, PFC delivery before ischemia worsens injury, highlighting the critical role of timing in PFC therapy for ischemia-reperfusion.

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Area of Science:

  • Biomedical Engineering
  • Physiology
  • Pharmacology

Background:

  • Perfluorocarbon (PFC) emulsions are investigated for their potential to mitigate ischemia-reperfusion injury.
  • The impact of PFC administration timing relative to ischemic events remains unclear.

Purpose of the Study:

  • To evaluate the differential effects of perfluorocarbon (PFC) emulsion administration before versus after an ischemic episode on microvascular function and leukocyte-endothelial cell interactions in a hamster window chamber model.
  • To determine the influence of PFC stagnation within the ischemic zone on these outcomes.

Main Methods:

  • Ischemia was induced in the hamster window chamber model for 1 hour.
  • Perfluorocarbon (PFC) emulsions or saline were administered either before or after ischemia.

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  • Intravital microscopy assessed vessel diameter, red blood cell velocity, leukocyte rolling and adhesion, and functional capillary density (FCD).

    • Main Results:

    • PFC administration after ischemia significantly reduced leukocyte rolling and adhesion and improved functional capillary density (FCD) and blood flow compared to saline controls.
    • Conversely, PFC administration before ischemia led to reduced FCD and increased leukocyte activation post-reperfusion.
    • These effects were linked to PFC stagnation in the ischemic zone when administered prior to the ischemic event.

    Conclusions:

    • Perfluorocarbon (PFC) administration after ischemia attenuates post-ischemic reperfusion injury by reducing leukocyte-endothelial cell interactions.
    • The timing of PFC administration is crucial, with post-ischemic delivery being beneficial and pre-ischemic delivery detrimental.
    • PFCs hold potential for managing ischemia-reperfusion injury, but their application requires careful consideration of administration timing to avoid adverse effects.