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Related Concept Videos

Upstream Processing01:27

Upstream Processing

97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
97

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Related Experiment Video

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Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
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PAK-in' up cGMP for the move.

Jeffrey Settleman1

  • 1MGH Cancer Center and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA. settleman@helix.mgh.harvard.edu

Cell
|January 27, 2007
PubMed
Summary

Guanylyl cyclases are activated by the Rac-p21-activated kinase (PAK) signaling pathway. This interaction promotes cell motility and migration by allosterically activating guanylyl cyclases.

Area of Science:

  • Cell biology
  • Molecular signaling
  • Biochemistry

Background:

  • Guanylyl cyclases are known to play a role in cell migration.
  • The precise mechanism of guanylyl cyclase activation in promoting cell motility remains unclear.

Purpose of the Study:

  • To elucidate the mechanism by which guanylyl cyclases are activated to promote cell motility.
  • To investigate the direct communication between guanylyl cyclases and the Rac-p21-activated kinase (PAK) signaling pathway.

Main Methods:

  • Investigated the interaction between guanylyl cyclases and the PAK signaling pathway.
  • Examined the role of autophosphorylated PAK in the allosteric activation of guanylyl cyclases.

Main Results:

  • Demonstrated direct communication between guanylyl cyclases and the Rac-p21-activated kinase (PAK) signaling pathway.

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Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
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Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

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  • Showed that autophosphorylated PAK allosterically activates guanylyl cyclases.
  • Confirmed that this pathway is essential for cell migration and motility.

    • Conclusions:

    • Autophosphorylated PAK directly activates guanylyl cyclases through allosteric mechanisms.
    • This novel signaling pathway is critical for regulating cell migration and motility.