Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Optimizing cell separation with the Cell Separator AS-106].

V Kretschmer1, C Rossa

  • 1Abt. Transfusionsmedizin und Gerinnungsphysiologie, Universitätsklinikum Marburg.

Beitrage Zur Infusionstherapie = Contributions to Infusion Therapy
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electric impedance spectroscopy feature extraction for tissue classification with electrode embedded surgical needles through a modified forward stepwise method.

Computers in biology and medicine·2021
Same author

RAP1-RAC1 Signaling Has an Important Role in Adhesion and Migration in HNSCC.

Journal of dental research·2020
Same author

Complexity of gap junctions between horizontal cells of the carp retina.

Neuroscience·2016
Same author

Effect of Aging on Periodontal Inflammation, Microbial Colonization, and Disease Susceptibility.

Journal of dental research·2016
Same author

Clopidogrel Enhances Mesenchymal Stem Cell Proliferation Following Periodontitis.

Journal of dental research·2015
Same author

Bacterial cellulose-hydroxyapatite composites with osteogenic growth peptide (OGP) or pentapeptide OGP on bone regeneration in critical-size calvarial defect model.

Journal of biomedical materials research. Part A·2015

Optimizing plateletpheresis protocols, specifically centrifugation speed, significantly enhances platelet yield and purity. This advancement improves the efficiency of collecting vital thrombocytes for transfusion and research applications.

Area of Science:

  • Hematology
  • Biotechnology
  • Medical Device Engineering

Background:

  • Plateletpheresis is a critical procedure for obtaining platelet concentrates.
  • Existing protocols have limitations in yield and purity, necessitating optimization.
  • Reducing leukocyte and erythrocyte contamination is crucial for transfusion safety.

Purpose of the Study:

  • To evaluate and optimize plateletpheresis separation protocols.
  • To identify key variables influencing thrombocyte yield and purity.
  • To minimize leukocyte and erythrocyte contamination in collected platelet products.

Main Methods:

  • Tested seventeen different separation protocols based on over 1100 plateletpheresis procedures.
  • Focused on optimizing centrifugation speed (1900 rpm at 50 ml/min blood flow).

Related Experiment Videos

  • Quantified thrombocyte yield and measured leukocyte and erythrocyte contamination levels.

    • Main Results:

    • Identified centrifugation speed as the most critical variable for platelet collection.
    • Achieved collection of 3.5-3.8 x 10^11 thrombocytes (approx. 60% extraction efficiency) from 3.15 L of blood.
    • Reduced leukocyte and erythrocyte contamination to approximately 1 x 10^7.

    Conclusions:

    • Optimized centrifugation parameters significantly improve thrombocyte yield and purity.
    • Further refinement is needed to address the 10% of thrombocytes remaining in the separation chamber.
    • The optimized protocol substantially reduces undesirable cellular contaminants, enhancing product quality.