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[A new procedure for lymphocyte depletion of thrombocyte concentrates with immunomagnetic beads]
B Häcker-Shahin1, A Karl, D J Giannitsis
1Blutspendezentrale, Städtischen Klinikums Karlsruhe.
In a series of pilot experiments we depleted platelet concentrates from contaminating leukocytes by use of immunmagnetic beads. The beads were coated with monoclonal antibodies directed against the leukocyte/lymphocyte antigens CD 2, CD 19 or CD 45. The amount of isolated cells depended on the dose of the used beads and was analogous to the observed decrease of leukocytes in platelet concentrates. The total amount of necessary beads for a complete leukocyte depletion depends on the grade of leukocyte contamination, which shows a high variation among different platelet preparations.
In a series of pilot experiments we depleted platelet concentrates from contaminating leukocytes by use of immunmagnetic beads. The beads were coated with monoclonal antibodies directed against the leukocyte/lymphocyte antigens CD 2, CD 19 or CD 45. The amount of isolated cells depended on the dose of the used beads and was analogous to the observed decrease of leukocytes in platelet concentrates. The total amount of necessary beads for a complete leukocyte depletion depends on the grade of leukocyte contamination, which shows a high variation among different platelet preparations.